Tsika Plate

Iyo sero tsika tsika ndiro inogona kuganhurwa kuita sandara pasi uye yakatenderera pasi (U-yakaumbwa uye V-yakaumbwa) zvichienderana nechimiro chepasi; huwandu hwematsime etsika ndi 6, 12, 24, 48, 96, 384, 1536, nezvimwe; zvinoenderana nezvakanyorwa, pane Terasaki ndiro uye yakajairwa maseru tsika ndiro. Iko kusarudzwa kwakatarwa kunoenderana nerudzi rwemaseru akakwenenzverwa, hunhu hwetsika hunodiwa uye zvakasiyana zvinangwa zvekuyedza.

(1) Musiyano nesarudzo yemapuratifomu-ezasi uye akatenderera-ezasi (U-akaumbwa uye V-akaumbwa) tsika mahwendefa

Maumbirwo akasiyana etsika tsika mahwendefa ane mashandisiro akasiyana Kune ekuvandudza maseru, yakadzika pasi inowanzo shandiswa, iri nyore kuti itariswe pasi pe microscope, ine yakajeka pasi nzvimbo, uye kukwirira kwesero tsika mvura mwero haina kuenderana. Naizvozvo, kana uchiedza zviyero zvakaita seMTT, angave ari anonamira kana akamiswa maseru, ndiro yakadzika yepasi inowanzo shandiswa. A-flat-bottomed culture plate inofanira kushandiswa kuyera kukosha kwekupinza. Chengetedza zvakanyanya izvo zvinhu. Iko kunzi "Tissue Tsika (TC) Inobatwa" ndeyechero tsika. Mahwendefa akaumbwa-U kana V-akaumbwa anowanzo shandiswa kana paine zvimwe zvakakosha zvinodiwa. Semuenzaniso, mune immunology, apo mairiymphocyte maviri akasiyana akasanganiswa tsika, iwo maviri anofanirwa kusangana kuti akurirane. Muchiitiko ichi, mahwendefa akafanana neU anowanzo shandiswa nekuti maseru anoungana munzvimbo diki nekuda kwesimba. Iyo yakakomberedzwa-yakadzika tsika ndiro inozoshandiswa zvakare kuyedza kweisotopu yekuisirwa, uye zvinodikanwa kushandisa mucheki unotora kuunganidza masero etsika, se "yakasanganiswa lymphocyte tsika" uye zvichingodaro. Mahwendefa akaumbwa-V anowanzo shandiswa pakuuraya maseru uye immune immune hemagglutination kuyedza. Iyo sero yekuuraya kuyedza inogona zvakare kutsiviwa neU-yakaumbwa ndiro (mushure mekuwedzera maseru, centrifugation pazasi kumhanya).

(2) Musiyano uripo pakati peTerasaki ndiro uye yakajairwa maseru tsika ndiro

Terasaki ndiro inowanzoshandiswa pakutsvaga kwekristalllographic, uye chigadzirwa chigadzirwa chiri nyore pakuongorora kwekristaro uye kuongorora kwechimiro. Kune nzira mbiri dzekugara nekutambidza donhwe, uye kuoneka uye chimiro chemashandisirwo ezvigadzirwa zveiyi nzira mbiri zvakare zvakasiyana. Sarudza kristal kirasi polymer senge chinhu, uye yakasarudzika zvinhu zvakanaka kucherechedza kristaro chimiro. Sero tsika ndiro yakanyanya kugadzirwa nePS zvinhu, uye izvo zvinhu zvinorapwa zvakakwana, izvo zviri nyore kuti sero kukura uye kuwedzera. Ehe, pane zvinhu zvekukura zveplanktonic maseru, pamwe neyepasi inosunga pamusoro.

(3) Musiyano uripo pakati pesero tsika ndiro uye microplate

Mahwendefa ane enzyme-akanyorwa anowanzo kudhura kupfuura maseru tsika tsika. Masero epamaseru anonyanya kushandiswa kune tsika tsika uye anogona zvakare kushandiswa kuyera mapuroteni kusangana. Mahwendefa akanyorwa enzyme anosanganisira kuputira mahwendefa uye mapindiro ekupindura. Kazhinji, ivo havafanirwe kushandiswa kune cell tsika. Ivo anonyanya kushandiswa kune immunoenzyme reaction. Gare gare kuwanikwa kweprotein kunoda zvakakwirira zvinodiwa uye chaiyo enzyme-yakanyorwa mvura yekushandisa.

(4) Iyo yepazasi nzvimbo yematsime etsika dzakasiyana mahwendefa uye huwandu hwakakurudzirwa hwemvura haufanire kunge hwakanyanya kudzika, kazhinji mukati meiyo 2 ~ 3mm, uye nzvimbo yepazasi yematsime akasiyana inogona kushandiswa kuverenga tsime rega rega. Chiyero chakaringana chemvura inofanirwa kuwedzerwa (tarisa patafura iri pazasi). Kana huwandu hwemvura yakawedzerwa yakawandisa, zvinokanganisa kuchinjaniswa kwegesi (okisijeni), uye zviri nyore kufashukira uye kukonzera kusvibiswa panguva yekufamba. Iyo chaiyo masero maseru ekuti awedzerwe zvinoenderana nechinangwa chekuyedza zvinoshanduka.